Left, two PhenoGraph meta-clusters present two distinct populations (1, 2) in Cluster#C. ( B) PhenoGraph defines two subpopulations of Cluster#C using mass cytometry (CyTOF). Results are representative of two independent experiments (n = 6 mice each). The expression patterns of the indicated markers are shown as histogram overlays of each cluster. Ly6G expression pattern is shown on viSNE map of Lin в€’CD117 + Ly6A/E в€’cells as spectrum colored dots. ViSNE maps of Lin в€’CD117 + Ly6A/E в€’cells are shown as dot overlays to display the 5 automated clusters (#A-E). BM cells isolated from C57BL/6J donors were stained with the antibody panel shown in Table S1. ( A) ViSNE defines a largest Cluster#C of the 5 subsets in Lin в€’CD117 + Ly6A/E в€’cells from murine BM using mass cytometry (CyTOF). Understanding the biology of hNeP should allow the development of new therapeutic targets for neutrophil-related diseases, including cancer.Д¬yTOF T cell suppression granulopoiesis melanoma neutrophil progenitor sarcoma scRNA-seq.Д¬opyright В© 2018 The Author(s). hNeP can be readily identified by flow cytometry and could be used as a biomarker for early cancer discovery. hNeP are present in the blood of treatment-naive melanoma patients but not of healthy subjects. NeP and hNeP both significantly increase tumor growth when transferred into murine cancer models, including a humanized mouse model. Both NeP and hNeP generate only neutrophils. Importantly, we found a similar unipotent NeP (hNeP) in human bone marrow. Here, we use cytometry by time of flight (CyTOF) and single-cell RNA sequencing (scRNA-seq) methodologies to identify a committed unipotent early-stage neutrophil progenitor (NeP) in adult mouse bone marrow. Neutrophils and monocytes originate from the granulocyte monocyte progenitor (GMP) in bone marrow however, unipotent neutrophil progenitors are not well defined. Neutrophils are short-lived cells that play important roles in both health and disease.

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